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. 2013 Nov 1;10:325. doi: 10.1186/1743-422X-10-325

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Copyright © 2013 Wang et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Strong interaction between P7-2 and SKP1^Maizein YTH system. Yeast colonies containing the combinations of pGBKT7-S7-2/pGADT7-SKP1^Maize and pGBKT7-S7-2/pGADT7-SKP1^rMaize grew well on the selective medium and turned blue, as did yeast colonies containing the positive control pGBKT7-p53/pGADT7-T. pGADT7-SKP1^rMaize is the prey clone from the positive transformants obtained in the two-hybrid screens, harboring the mRNA sequence of SKP1, while pGADT7-SKP1^Maize is the construct that expresses the exact SKP1^Maize protein. Yeast transformed with pGBKT7-S7-2/pGADT7 or pGBKT7/pGADT7-SKP1^Maize used as negative controls were unable to grow.