Figure 2.

BiFC visualization of P7-2-SKP1^Maize interaction in agrobacterium-infiltrated N. benthamiana leaves. Co-expression of P7-2-YN/SKP1^Maize-YC or P7-2-YC/SKP1^Maize-YN induced a weak fluorescence pattern. The signals were localized in or at the peripheries of the nuclei and the plasma membrane, and slight diffuse fluorescence was also observed in the cytoplasm. No YFP signals were detected for the negative controls. YFP was excited at 488 nm and emission was measured at 550–590 nm. The fluorescent, bright field and merged images are depicted in the upper, middle and bottom panels, respectively. Bars, 20 μm.