Figure 5.

Mapping of the crucial region of P7-2 involved in the interaction with SKP1^Maize. (A) Schematic representation of P7-2 and its truncations in the study. The full-length P7-2 (spanning residues 1 to 309) and its truncations are indicated by blue bars while the deleted regions by dashed lines. The predicted α-helix and the short sequence (LPFAELL) similar to F-box consensus sequence (LPxxI/L) were also indicated. The numbers denote the amino acid positions of the proteins. The ability of different P7-2 truncations to interact with SKP1^Maize is indicated on the right (+, positive; -, negative). (B) The interaction between different P7-2 truncations and SKP1^Maize in YTH assays. Yeast colonies expressing AD-SKP1^Maize with BD-P7-2^25-309, BD-P7-2^44-309, BD-P7-2^79-309, and P7-2-LP^(79–80)AA were able to grow well on the selective medium and turned blue. BD-P7-2^1-295 and BD-P7-2^1-214 showed decreased capability to interact with SKP1^Maize. No growth was observed in the yeast expressing AD-SKP1^Maize with BD-P7-2^1-287 and BD-P7-2^79-214.