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. 2013 Oct 25;13:282. doi: 10.1186/1472-6882-13-282

Table 1.

Sequences of primers and PCR conditions for semi-quantitative RT-PCR

Gene Sequence (5′-3′) Annealing temperature (°C) Size (bp) Accession No.
ADRP
 
55
352
NM_00748
Sense
CTT GTG TCC TCC GCT TAT GTC AGT
Anti-sense
CTG CTC CTT TGG TCT TAT CCA CCA
Perilipin
 
55
508
NM_175640
Sense
CTT TCT CGA CAC ACC ATG GAA ACC
Anti-sense
CCA CGT TAT CCG TAA CAC CCT TCA
PPAR-γ
 
55
220
NM_011146
Sense
CCA GAG CAT GGT GGG TTC GCT G
Anti-sense
GAG CTG ACC CAA TGG TTG CTG
C/EBP-α
 
55 238 NM_007678
Sense
AGG TGG TGG AGT TGA CCA GT
Anti-sense CAG CCT AGA GAT CCA GCG AC