Fig. 3. Binding of CRP to PnC.

Microtiter wells were coated with PnC. Culture media containing various CRP species were diluted to ~50 ng/ml in TBS-Ca buffer and added to the wells. Bound CRP was detected using the anti-CRP mAb HD2.4 as reporter. Values on the x axis represent the ratio of the concentration of CRP measured by the PnC binding assay over that measured by ELISA run in parallel using nCRP to generate standard curves in both assays.