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. 2013 Oct 28;13:169. doi: 10.1186/1471-2229-13-169

Figure 5.

Figure 5

Expression of wild type CYP709B3 gene incyp709b3mutant can rescue salt intolerance phenotype. A-D. Expression pattern of ProCYP709B3:GUS in different organs. The results of GUS staining were observed in: seedling (A), leaf (B), silique (C) and flower (D). E. Expression of a wild type CYP709B3 gene can rescue the salt intolerance phenotype. Wild type and three independent ProCYP709B3:CYP709B3 transgenic homozygous lines (11–4, 18–8 and 40–2) in cyp709b3 mutant background were treated by 150 mM NaCl. The seedlings were counted at the indicated times. F. Analysis of the expression level of CYP709B3 in seedlings from wild type, cyp709b3 and independent transgenic homozygous lines (11–4, 18–8 and 40–2). ACTIN2 was used as internal control. Values are the means ± SE of three replicates. Statistically different (p value < 0.05) is indicated using asterisks.