Figure 6.

Gene expression analysis in wild type and mutants under 150 mM NaCl treatment. Seeds were germinated on MS medium for 4 days and transferred to MS medium supplemented with 150 mM NaCl. Seedlings were harvested at indicated times. Gene expression was detected by quantitative real time PCR. A.CYP709B3 and CYP709B2 gene expression in wild type seedlings under 150 mM NaCl treatment. Expression of stress response genes: KIN2(B), RD29A(C), RD29B(D), DREB1A(E), ERD10(F) in seedlings from 150 mM NaCl-treated wild type and cyp709b3 mutant. ACTIN2 was used as internal control. Error bars indicate SE (n = 3). Statistically different (p value < 0.05) is indicated using asterisks.