Table 2.
Differential expression of genes related to the immune response
| Gene ID 1 | Annotation 2 | Mean fold change± SE 3 | Identity 4 |
|---|---|---|---|
| Ssa#S24188435 |
AY929386 |
216.3 ± 58.8 |
TNFα 2 |
| Omy#gi185133433 |
NM_001124347 |
197.9 ± 79.1 |
IL-1B |
| Ssa#STIR00083_4 |
AY929385 |
99.2 ± 17.9 |
TNFα 1 |
| Omy#gi13235345 |
AJ279069 |
93.3 ± 15.4 |
IL-8 |
| Ssa#S34822137 |
AM397592 |
29.7 ± 5.1 |
Complement c3 |
| Ssa#STIR04816 |
BT047247 |
25.5 ± 13.1 |
Hepcidin |
| Omy#S37211068 |
EF175381 |
20.7 ± 1.1 |
Prostaglandin G/H synthase 2b |
| Ssa#STIR08688 |
TC65065 |
17.9 ± 4.9 |
Ferritin |
| Ssa#S21512941 |
AY572832 |
16.1 ± 0.2 |
C type lectin receptor A |
| Ssa#STIR35259 |
NM_001124618 |
13.8 ± 0.9 |
Complement protein component c7-1 |
| Ssa#STIR23928 |
NM_001124410.1 |
13.3 ± 10.8 |
Complement factor H precursor |
| Ssa#S43134841_S |
NM_001123611 |
9.5 ± 2.5 |
CD4-like protein |
| Ssa#STIR00084_4 |
DW569632 |
8.5 ± 0.3 |
NF-kappa-b inhibitor alpha |
| Ssa#S18892257 |
AJ505008 |
3.9 ± 0.3 |
IL-1 receptor accessory protein |
| Ssa#STIR14647 |
TC73172 |
3.9 ± 0.0 |
MAP kinase-interacting serine/threonine kinase 2 |
| Ssa#S30276405 |
DW563373 |
3.9 ± 0.2 |
Suppressor of cytokine signaling 1 |
| Ssa#S35660755 |
EG895473 |
3.4 ± 0.3 |
NF-kappa-B p100 subunit |
| Ssa#S35667643 |
EG902361 |
3.4 ± 0.2 |
Complement c1q-like protein 4 |
| Ssa#S31992293 |
DY720890 |
3.2 ± 0.2 |
IL-10 receptor beta chain precursor |
| Omy#gi197927463 |
NM_001124396 |
3.0 ± 0.2 |
IL-1 receptor antagonist |
| Ssa#KSS392 |
NM_001141766 |
2.9 ± 0.3 |
IL-1 receptor-associated kinase 4 |
| Ssa#CB516003 |
CB516003 |
2.8 ± 0.3 |
NF-kappa-B 1 p105 subunit |
| Ssa#KSS3660 |
BT059477 |
2.8 ± 0.3 |
NF-kappa-B inhibitor epsilon |
| Ssa#STIR08793 |
TC65192 |
2.8 ± 0.1 |
Suppressor of cytokine signaling 3 |
| Ssa#STIR00087_4 |
DW555246 |
2.7 ± 0.2 |
IL-10 |
| Ssa#STIR12117 |
TC69580 |
2.3 ± 0.1 |
Transcription factor jun-B |
| Omy#S18157537 |
BX883008 |
2.1 ± 0.1 |
Interleukin-6 receptor subunit alpha precursor |
| Ssa#DW006091 |
DW006091 |
2.1 ± 0.2 |
MHC class i antigen |
| Ssa#TC106540 |
TC106540 |
−2.4 ± 0.1 |
TNF receptor-associated factor 6 |
| Ssa#STIR08822 |
TC65229 |
−2.6 ± 0.1 |
IL-15 |
| Ssa#S18849636_S |
BT071912.1 |
−2.6 ± 0.2 |
Complement C4-1 |
| Ssa#S35693513 |
EG928231 |
−3.3 ± 0.8 |
Complement component 6 precursor |
| Ssa#S35687715 | EG922433 | −22.7 ± 8.3 | Complement factor D precursor |
List of selected mRNAs associated with the immune response found to be increased or decreased in expression in response to rIL-1β stimulation. Genes were assigned to the table based upon both their GO identifier and previous knowledge of their functions. Genes with greatest fold differences in expression are presented, the genes that are lower in expression are denoted by (-) value. The genes shown were significant at p < 0.05 following t- tests with Benjamini-Hochberg FDR and greater than 2-fold change. 1Indicates the unique code for the feature on the microarray, 2Accession number of the cDNA sequence, accession numbers beginning with TC are for oligos from the TIGR Atlantic Salmon Gene Index. 3Fold change, in the case of oligos that featured multiple times in the gene list the one with the highest fold change is reported. 4Identity of the probe target as determined by BLASTX and BLASTN searches.