Table 7.
Primers used for qPCR
| Name 1 | Accession 2 | Sequence 5′ to 3′ | bp 3 | |
|---|---|---|---|---|
| EF-1α |
AF498320.1 |
F |
CAAGGATATCCGTCGTGGCA |
327 |
| |
|
R |
ACAGCGAAACGACCAAGAGG |
|
| RPL1 |
CB516726 |
F |
ACTATGGCTGTCGAGAAGGTGCT |
118 |
| |
|
R |
TGTACTCGAACAGTCGTGGGTCA |
|
| HPRT1 |
EG866745 |
F |
CCGCCTCAAGAGCTACTGTAAT |
255 |
| |
|
R |
GTCTGGAACCTCAAACCCTATG |
|
| IL-1β |
AJ223954.1 |
F |
GCTGGAGAGTGCTGTGGAAGAACATATAG |
179 |
| |
|
R |
CCTGGAGCATCATGGCGTG |
|
| Hepcidin |
NM_001140849.1 |
F |
CATTGAAAATCGTGCATTGG |
150 |
| |
|
R |
AGGCCTTCATTCTCGGTTTT |
|
| IGFBP-6 |
DQ190459.2 |
F |
GCTCAATAGTGTTCTGCGTGG |
118 |
| |
|
R |
CTTGGAGGAACGACACTGCTT |
|
| TNFα1 |
NM_001123589.1 |
F |
TGTGTGGCGTCCTCTTAGTAGCAGCTT |
101 |
| |
|
R |
CTCCATTTTGTCCTGCATCGTTGC |
|
| IGFBP-4 |
EU861007 |
F |
TGTCGTGCTGAGCTGCAGAG |
129 |
| |
|
R |
TGGCTGGCACTGCTTGGCAT |
|
| IGFBP-5.2 |
EU861009 |
F |
TTCTCCAGAGGAAGCTATGTTAG |
170 |
| |
|
R |
TCAAGGCTGCTGACAGAGTG |
|
| Myf5 |
TC97553 |
F |
CGCATACCGCTTTTACTTCC |
245 |
| |
|
R |
TGATCATGAGAAACGTGAAGC |
|
| High choriolytic enzyme |
TC63579 |
F |
ATCAATGGGGCTCATCTCAG |
239 |
| |
|
R |
ATGAGCAAACACGCAGTGAC |
|
| Atrogin-1 |
GU456729.1 |
F |
CGAGTGCTTCCAGGAGAATCTG |
384 |
| R | GTCTGAAGGAGCTCCTTGATGG |
List of primers used in qPCR, all primers were checked to ensure that they had an efficiency of between 1.8 & 2.0. All except for Atrogin 1 were used for qPCR confirmation of the microarray.1Identity of the gene, 2 Accession number of the cDNA sequence, 3Size of PCR product produced by the primers.