Fig. 4. Effect of SB-657510 (SB) on urotensin II (UII)-induced expression of tissue factor (TF). (A) RT-PCR of TF after UII treatment. EA.hy926 cells were treated with 100 nM UII for 0.5 or 1 h. n=4. (B) RT-PCR of TF after UII and SB treatment. After pretreatment with 1 μM SB, cells were treated with 100 nM UII for 1 h in the presence or absence of SB. Quantitative analysis is shown in the bottom. *p<0.05 vs. control (CTL), #p<0.05 vs. vehicle (Veh). n=4. (C) Western blots of TF after UII and SB treatment. After pretreatment with 1 μM SB, cells were treated with 100 nM UII for the indicated times in the presence or absence of SB. Quantitative analysis is shown in the bottom. *p<0.05 vs. 0 h, #p<0.05 vs. UII. n=4.
