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. Author manuscript; available in PMC: 2013 Nov 7.
Published in final edited form as: J Mol Biol. 2011 Sep 6;413(3):10.1016/j.jmb.2011.08.057. doi: 10.1016/j.jmb.2011.08.057

Figure 2.

Figure 2

Engineering a salt bridge in β1AR-m23. (a) The structure of β1AR-m23 with D200 and D322 in space-filling representation (C, green; O, red; N, blue) is compared to (b) β2AR where the corresponding residues, D192 and K305 form a salt bridge. (c) Apparent thermostability of various salt bridge forming mutations: β1AR-m23 (blue circles); D200E (orange squares); D322K (green triangles); D322R (cyan inverted triangles); D200E+D322K (purple diamonds); D200E+D322R (pink stars). Data points are from duplicate measurements in a representative experiment with error bars representing the SEM. (d) Change in the apparent Tm of the salt bridge mutants relative to β1AR-m23 measured in DDM and NG (±SEM).