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(a) Thermostability changes of leucine mutations and combinations of mutations compared to β1AR-m23 (solid baseline). The stability of each single mutant relative to β1AR-m23 is shown (blue bars) adjacent to the stability of each double mutant i.e. the mutant indicated plus Y343L (red bars). The dashed line at 5°C indicates the stability of β1AR-m23+Y343L. (b) Thermostability of β1AR-m23 (blue circles) and β1AR-JM3 (green triangles) in NG.
