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. Author manuscript; available in PMC: 2013 Nov 7.
Published in final edited form as: J Mol Biol. 2011 Sep 6;413(3):10.1016/j.jmb.2011.08.057. doi: 10.1016/j.jmb.2011.08.057

Figure 6.

Figure 6

Thermostability of β1AR-JM3 in different detergents. (a) E. coli membranes containing β1AR-JM3 were solubilised in the respective detergents and the apparent Tms determined from the thermostability curves: dodecylmaltoside (black empty squares), Tm 61.7 ± 0.9°C; decylmaltoside (grey empty triangles), Tm 52.8 ± 0.5°C; nonylglucoside (red circles), Tm 34.5 ± 0.7°C; octylglucoside (orange squares), Tm 26.8 ± 0.2°C; heptylthioglucoside (green triangles), Tm 22.5 ± 0.7°C; fos-choline 9 (blue inverted triangles), Tm 35.3 ± 0.5°C; SDS (purple diamonds), Tm 33.0 ± 0.7°C; LDAO (pink hexagons), Tm 31.6 ± 0.3°C; CYMAL-3 (brown stars), Tm 33.8 ± 0.5°C; DHPC (cyan empty circles), Tm 37.4 ± 1.0°C. Detergent concentrations were all 2% except for SDS (1%) and LDAO (0.9%). Data are from a single experiment performed simultaneously in duplicate with errors representing the SEM. (b) Comparison of the stability of β1AR-JM3 with β1AR-m23 and wild-type β1AR (data for β1AR-m23 and β1AR are from reference 23). Symbols refer to the graph in panel (a).