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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: Anal Chem. 2013 Sep 23;85(20):10.1021/ac401978w. doi: 10.1021/ac401978w

Figure 1.

Figure 1

(a) CID tandem mass spectrum of the [M+4H+TMT6]4+ ion of the peptide fragment SRHPAENGKSNF, acquired using the PAN mode (see experimental section for details). A series of unmodified b ions from b2 to b8, modified b9 b10 and b11 ions, unmodified y2 and modified y4 and y6 ions confirm that Lys19 is the site of modification. The product ions with an asterisk are the product ions that contain the TMT6 modification. The inset is an enlarged view of the reporter ion region. (b) Dose-response plots for the peptide fragment (SRHPAENGKSNF) obtained by plugging the reporter ion abundances at m/z’s 126 to 131 into equation 8. The k value is obtained from the slope and is corrected using equation 2.