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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: Anal Chem. 2013 Sep 23;85(20):10.1021/ac401978w. doi: 10.1021/ac401978w

Figure 3.

Figure 3

(a) CID mass spectrum of the [M+4H+TMT6]4+ ion of the peptide fragment KNGERIEKVEHSDL, acquired using the PAN mode (see experimental section for details). A series of modified b3 to b13 ions, and a series of unmodified y2 to y12 ions confirm that Lys41 is the site of modification. The inset is an enlarged view of the reporter ion region. (b) ETD mass spectrum of the [M+4H+TMT6]4+ ion of the peptide fragment KNGERIEKVEHSDL. A series of modified ions from c1 to c13 and a series of unmodified ions from z4 to z13 confirm that Lys41 is the site of modification by TMT6. The inset is an enlarged view of the reporter ion region. In all spectra, the product ions with an asterisk are the product ions that contain the TMT modification.