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. 2013 Dec;25(12):2511–2517. doi: 10.1016/j.cellsig.2013.08.013

Fig. 1.

Fig. 1

TOPK is a novel CHFR associated protein. (a) 293 T cells were transfected with Flag-tagged TOPK (upper panel) or Flag-tagged CHFR (lower panel). The interaction of CHFR and TOPK was detected by immunoblotting with anti-CHFR and anti-TOPK antibodies after immunoprecipitation with anti-IgG and anti-Flag antibodies. (b) The in vitro interaction of endogenous TOPK with CHFR was detected by immunoblotting with anti-TOPK antibody after performing GST pull down assay with either GST or GST–CHFR using 293 T cell lysate. (c) Schematic representation of domain architecture of full length CHFR, along with its various deletion mutants. (d) 293 T cells were co-transfected with the indicated Myc-tagged CHFR constructs along with Flag-tagged TOPK and the interaction was determined by immunoprecipitation and immunoblotting with the indicated antibodies. (e) Schematic representation of N-terminal Flag-tagged full-length TOPK, along with its various deletion mutants. (f) 293 T cells were co-transfected with the indicated Flag-tagged TOPK constructs along with those encoding Myc tagged CHFR and the interaction between TOPK and CHFR was determined by immunoprecipitation and immunoblotting with the indicated antibodies.