Table 1.

Optimization of the performance of the artificial benzannulase.

entry	Sav Mutant	Solvent	yield (%)*	regioisomeric ratio (rr)	enantiomeric ratio (er)
1	-	Acetate Buffer	80	4:1	51.5:48.5
2	-	H2O	< 5%	-	-
3	WT†	H2O	< 5%	-	-
4	WT†	Acetate Buffer	46	9:1	75:25
5	S112E†	H2O	10	15:1	78:22
6	S112D†	H2O	< 5%	-	-
7	K121E†	H2O	7	15:1	78:22
8	K121D	H2O	89	15:1	78:22
9	K121N†	H2O	< 5%	-	-
10	N118K-K121E	H2O	99	15:1	82:18
11	N118K-K121E†,‡	H2O	8	6:1	52:48
12	S112A	Acetate Buffer	12	6:1	75:25
13	S112C	Acetate Buffer	6	10:1	71:29
14	S112F	Acetate Buffer	50	6:1	86:14
15	S112K	Acetate Buffer	6	6:1	76:24
16	S112M	Acetate Buffer	1	6:1	81:19
17	S112T	Acetate Buffer	35	6:1	79:21
18	S112V	Acetate Buffer	4	5:1	81:19
19	S112Y	Acetate Buffer	30	12:1	88:12
20	S112W	Acetate Buffer	32	12:1	86:14
21	S112Y-K121D	H2O	30	20:1	90:10
22	S112Y-K121E	H2O	80	20:1	90:10
23	S112Y-K121E	MOPS Buffer	95	19:1	91:9

^*Yield determined by gas chromatography integration

^†Reaction conducted for 36 h.

^‡Sav preloaded with excess biotin for 10 min and then treated with [RhCp*^biotinCl₂]₂.