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Assessing the in vivo stability of the pH sensitive two-stranded I-switch, ITfA488/A647. Plots showing (a) mean acceptor intensity per cell and (b) mean donor/FRET (D/A) ratio as a function of time. Error bars indicate s.e.m. (n = 10 worms, ⩾20 cells) (c) Representative images of coelomocytes labeled with ITfA488/A647 at various time points. Scale bar: 5 μm.
