Table 2.
Amino acid residues in MDCK cell- and egg-cultured viruses and viruses in clinical specimens.
| Virus isolates and clinical samples (day since onset of illness) | HA position |
Amino acid (%)a |
|
|---|---|---|---|
| Wild-typeb | Mutant | ||
| A/VN/CL1/04 | 186 | Glu (5) | Asp (95) |
| A/VN/CL1/04 ω5 | 186 | Glu (97) | Asp (3) |
| CL1 Throat swab (d8)c | 186 | Glu (100) | Asp (0) |
| CL1 Nose swab (d10) | 186 | Glu (100) | Asp (0) |
| A/VN/CL2009C/05 | 151 | Ile (3) | Phe (95), Leu (2) |
| A/VN/CL2009C/05 ω5 | 151 | Ile (0) | Phe (2), Leu (98) |
| CL2009 Throat swab (d7) | 151 | Ile (35) | Phe (65) |
| CL2009 Nose swab (d8) | 151 | Ile (0) | Phe (100) |
| CL2009 Throat swab (d8)c | 151 | Ile (0) | Phe (100) |
| A/VN/CL105/05 | 134 | Ala (48) | Val (52) |
| A/VN/CL105/05 ω5 | 134 | Ala (96) | Val (4) |
| CL105 Throat swab (d4)c | 134 | Ala (26) | Val (74) |
| CL105 Tracheal aspirate (d4) | 134 | Ala (27) | Val (73) |
ω5: five serial passages in the allantois of embryonated chicken eggs.
a
Proportions of subpopulations were determined by pyrosequencing of the regions of interest.
b
Wild-type refers to the avian virus consensus sequence.
c
Source of the MDCK cell isolate.