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. Author manuscript; available in PMC: 2014 Jul 27.
Published in final edited form as: Neuroimmunomodulation. 2013 Jul 27;20(5):10.1159/000351610. doi: 10.1159/000351610

Table 1.

Summary of experimental protocols tested. (PBMC = peripheral blood mononuclear cell)

Experiment
(Figure #)		 Participants Cell type LPS dose
(ng/mL)		 OT doses
(pM)		 Pre-incubation
with OT (min)		 Cytokine
Measurement
1 (1) Female Monocytes (whole blood) 50 0, 1, 10, 100, 1000 0 FACS (CD14+ /IL-6 +)
Male Monocytes (whole blood) 50 0, 1, 10, 100, 1000 0 FACS (CD14+ /IL-6+)
2 (1) Female Monocytes (whole blood) 50 0, 1, 10, 100, 1000 0 FACS (CD14+ /IL-6 +)
Monocytes (whole blood) 50 0, 1, 10, 100, 1000 30 FACS (CD14+ /IL-6 +)
Female Monocytes (whole blood) 50 0, 1, 10, 100, 1000 30 FACS (CD14+ /IL-6 +)
Monocytes (whole blood) 50 0, 1, 10, 100, 1000 60 FACS (CD14+ /IL-6 +)
3 (1) Male Isolated PBMCs 50 0, 1, 10, 100, 1000 30 FACS (CD14+ IL-6 +)
Female Isolated PBMCs 50 0, 1, 10, 100, 1000 30 FACS (CD14+ IL-6 +)
4 (2) Female Monocytes (whole blood) 50 0, 1, 10, 100, 1000 0 ELISA (IL-6)
Monocytes (whole blood) 0.5 0, 1, 10, 100, 1000 0 ELISA (IL-6)
5 (3) Male Monocytes (whole blood) 50 0, 1, 10, 100 30 Meso Scale (TNFα, IL-6, IL-8, IL-1β)
6 (2) Male Monocytes (whole blood) 50 0, 0.1, 1, 10, 100, 1000, 10000 30 ELISA (IL-6)
Female Monocytes (whole blood) 50 0, 0.1, 1, 10, 100, 1000, 10000 30 ELISA (IL-6)
Female Monocytes (whole blood) 50 0, 0.1, 1, 10, 100, 1000, 10000 30 ELISA (IL-6)
7 (2, 4) Male Macrophages 50 0, 0.1, 10, 100, 1000 0 ELISA (TNFα); Mesoscale (TNFα, IL-6, IL-8, IL-1β)
Male Macrophages 50 0, 0.1, 10, 100, 1000 30 ELISA (TNFα); Mesoscale (TNFα, IL-6, IL-8, IL-1β)
Male Macrophages 50 0, 0.1, 10, 100, 1000 0 Mesoscale (TNFα, IL-6, IL-8, IL-1β)
Male Macrophages 50 0, 0.1, 10, 100, 1000 30 Mesoscale (TNFα, IL-6, IL-8, IL-1β)