Table 1.
Strain/population | Relevant characteristics | MGEa | ST donor | Reference |
---|---|---|---|---|
64/3 | recipient plasmid-free E. faecium strain: RifR, FusR | — | ST21 | 35 |
64/3PEV | recipient E. faecium strain 64/3 serially transferred for 400 generations in BHI broth resulting in a mixed evolved ancestral population: RifR, FusR | — | ST21 | this study |
64SS | recipient plasmid-free E. faecium strain: StrR, SptR | — | ST21 | 35 |
OG1RF | recipient plasmid-free E. faecalis strain: RifR, FusR | — | ST1 | 8 |
70/90 | clinical E. faecium strain from 1990 (France) | 100 kb rep-inc18 vanA plasmid with axe-txe (PSK) + 45 kb plasmid | ST25 | 35 |
UW3540 | clinical E. faecium strain from 2002 (Germany) | 80 kb rep-inc18 vanA-erm(B)- vat(D) plasmid | ST145 | 35 |
H182 | clinical E. faecium strain from 2002 (Portugal) | 92 kb rep-pRUM vanA plasmid | ST18 | 35 |
UW261 | food E. faecium strain from 1995 (Germany) | 172 kb vanA plasmid of unknown type with ω-ɛ-ζ (PSK) | ND | 35 |
E0292 | clinical E. faecium strain from 2002 (USA) | 200 kb rep-inc18 vanA plasmid | ST20 | 35 |
229710 | clinical E. faecalis strain from 1996 (Portugal) | 85 kb rep-inc18/rep-pAD1 vanA plasmid with par | ST6 | 35 |
664.1H1 | E. casseliflavus donor of Tn6000: TetR | 33 kb CTn (Tn6000) | — | 33 |
BS34A | B. subtilis donor of Tn916: TetR | 18 kb CTn (Tn916) | — | 32 |
TC1Tn6000 | transconjugant E. faecium 664.1H1 × 64/3: RifR, FusR, TetR | 33 kb Tn6000 | — | this study |
TC2Tn916 | transconjugant E. faecium BS34A × 64/3: RifR, FusR, TetR | 18 kb Tn916 | — | this study |
TC3 | transconjugant E. faecium UW3114 × 64/31 with plasmid pLG2, PAI(–): EryR, FusR, RifR | pLG2 | ST21 | 8 |
TC4PAI | transconjugant E. faecium UW3114 × 64/32 with plasmid pLG2, PAI(+): EryR, FusR, RifR | pLG2, 200 kb PAI | ST21 | 8 |
TC5 | transconjugant E. faecalis UW3114 × OG1RF1 with plasmid pLG2, PAI(–): EryR, FusR, RifR | pLG2 | ST1 | 8 |
TC6PAI | transconjugant E. faecalis UW3114 × OG1RF2 with plasmid pLG2, PAI(+): EryR, FusR, RifR | pLG2, 200 kb PAI | ST1 | 8 |
TCvanA | transconjugant E. faecium 70/90 × 64/3: VanR, FusR, RifR | 100 kb vanA- plasmid + 45 kb plasmid | ST21 | 35 |
TC8vanA | transconjugant E. faecium UW3540 × 64/3: VanR, FusR, RifR | 80 kb vanA plasmid | ST21 | 35 |
TC9vanA | transconjugant E. faecium H182 × 64/3: VanR, FusR, RifR | 92 kb vanA plasmid | ST21 | 35 |
TC10vanA | transconjugant E. faecium UW261 × 64/3: VanR, FusR, RifR | 172 kb vanA plasmid | ST21 | 35 |
TC11vanA | transconjugant E. faecium E0292 × 64/3: VanR, FusR, RifR | 200 kb vanA plasmid | ST21 | 35 |
TC12vanA | transconjugant E. faecium 229710 × 64/3: VanR, FusR, RifR | 85 kb vanA plasmid | ST21 | 35 |
TC7 -1PvanA
EV TC7 -2PvanA EV |
transconjugants E. faecium 70/90 × 64/3 serially transferred for 400 generations in BHI broth without antibiotics resulting in mixed evolved populations: VanR, FusR, RifR | 100 kb vanA plasmid | ST21 | this study |
TC10-1PvanA
EV TC10-2PvanAEV TC10-3PvanA EV |
transconjugants E. faecium UW261 × 64/3 serially transferred for 400 generations in BHI broth without antibiotics resulting in mixed evolved populations: VanR, FusR, RifR | 172 kb vanA plasmid | ST21 | this study |
TC12-1PvanAEV TC12-2PvanAEV TC12-3PvanA EV |
transconjugants E. faecium 229710 × 64/3 serially transferred for 400 generations in BHI broth without antibiotics resulting in mixed evolved populations: VanR, FusR, RifR | 85 kb vanA plasmid | ST21 | this study |
TC7-1vanAEV TC7-2vanA EV |
fitness-representative strains of the evolved populations TC7PEV: VanR, FusR, RifR | 100 kb vanA plasmid | ST21 | this study |
TC10-1vanA
EV TC10-2vanA EV TC10-3vanA EV |
fitness-representative strains of the evolved populations TC10PEV: VanR, FusR, RifR | 172 kb vanA plasmid | ST21 | this study |
TC12-1vanA
EV TC12-2vanA EV TC12-3vanA EV |
fitness-representative strains of the evolved populations TC12PEV: VanR, FusR, RifR | 85 kb vanA plasmid | ST21 | this study |
TC7vanASS | transconjugant E. faecium (70/90 × 64/3) × 64SS: VanR, StrR, SptR | 100 kb vanA plasmid | ST21 | this study |
TC10vanASS | transconjugant E. faecium (UW261 × 64/3) × 64SS: VanR, StrR, SptR | 172 kb vanA plasmid | ST21 | this study |
TC12vanASS | transconjugant E. faecium (229710 × 64/3) × 64SS: VanR, StrR, SptR | 85 kb vanA plasmid | ST21 | this study |
Van, vancomycin; Ery, erythromycin; Str, streptomycin (high level of resistance); Spt, spectinomycin (high level of resistance); Fus, fusidic acid; Rif, rifampicin; Tet, tetracycline; ND, not determined; PSK, post-segregation killing system.
aPlasmid content present in both donors and transconjugants is described. Several donors contained more than one plasmid; see Werner et al.35