. 2013 Jul 5;68(12):2755–2765. doi: 10.1093/jac/dkt270

Table 1.

Strains and populations used in this study

Strain/population	Relevant characteristics	MGE^a	ST donor	Reference
64/3	recipient plasmid-free E. faecium strain: Rif^R, Fus^R	—	ST21	35
64/3^P_EV	recipient E. faecium strain 64/3 serially transferred for 400 generations in BHI broth resulting in a mixed evolved ancestral population: Rif^R, Fus^R	—	ST21	this study
64SS	recipient plasmid-free E. faecium strain: Str^R, Spt^R	—	ST21	35
OG1RF	recipient plasmid-free E. faecalis strain: Rif^R, Fus^R	—	ST1	8
70/90	clinical E. faecium strain from 1990 (France)	100 kb rep-inc18 vanA plasmid with axe-txe (PSK) + 45 kb plasmid	ST25	35
UW3540	clinical E. faecium strain from 2002 (Germany)	80 kb rep-inc18 vanA-erm(B)- vat(D) plasmid	ST145	35
H182	clinical E. faecium strain from 2002 (Portugal)	92 kb rep-pRUM vanA plasmid	ST18	35
UW261	food E. faecium strain from 1995 (Germany)	172 kb vanA plasmid of unknown type with ω-ɛ-ζ (PSK)	ND	35
E0292	clinical E. faecium strain from 2002 (USA)	200 kb rep-inc18 vanA plasmid	ST20	35
229710	clinical E. faecalis strain from 1996 (Portugal)	85 kb rep-inc18/rep-pAD1 vanA plasmid with par	ST6	35
664.1H1	E. casseliflavus donor of Tn6000: Tet^R	33 kb CTn (Tn6000)	—	33
BS34A	B. subtilis donor of Tn916: Tet^R	18 kb CTn (Tn916)	—	32
TC1_Tn6000	transconjugant E. faecium 664.1H1 × 64/3: Rif^R, Fus^R, Tet^R	33 kb Tn6000	—	this study
TC2_Tn916	transconjugant E. faecium BS34A × 64/3: Rif^R, Fus^R, Tet^R	18 kb Tn916	—	this study
TC3	transconjugant E. faecium UW3114 × 64/3₁ with plasmid pLG2, PAI(–): Ery^R, Fus^R, Rif^R	pLG2	ST21	8
TC4_PAI	transconjugant E. faecium UW3114 × 64/3₂ with plasmid pLG2, PAI(+): Ery^R, Fus^R, Rif^R	pLG2, 200 kb PAI	ST21	8
TC5	transconjugant E. faecalis UW3114 × OG1RF₁ with plasmid pLG2, PAI(–): Ery^R, Fus^R, Rif^R	pLG2	ST1	8
TC6_PAI	transconjugant E. faecalis UW3114 × OG1RF₂ with plasmid pLG2, PAI(+): Ery^R, Fus^R, Rif^R	pLG2, 200 kb PAI	ST1	8
TC_vanA	transconjugant E. faecium 70/90 × 64/3: Van^R, Fus^R, Rif^R	100 kb vanA- plasmid + 45 kb plasmid	ST21	35
TC8_vanA	transconjugant E. faecium UW3540 × 64/3: Van^R, Fus^R, Rif^R	80 kb vanA plasmid	ST21	35
TC9_vanA	transconjugant E. faecium H182 × 64/3: Van^R, Fus^R, Rif^R	92 kb vanA plasmid	ST21	35
TC10_vanA	transconjugant E. faecium UW261 × 64/3: Van^R, Fus^R, Rif^R	172 kb vanA plasmid	ST21	35
TC11_vanA	transconjugant E. faecium E0292 × 64/3: Van^R, Fus^R, Rif^R	200 kb vanA plasmid	ST21	35
TC12_vanA	transconjugant E. faecium 229710 × 64/3: Van^R, Fus^R, Rif^R	85 kb vanA plasmid	ST21	35
TC7 -1^P_vanA _EV TC7 -2^P_vanA _EV	transconjugants E. faecium 70/90 × 64/3 serially transferred for 400 generations in BHI broth without antibiotics resulting in mixed evolved populations: Van^R, Fus^R, Rif^R	100 kb vanA plasmid	ST21	this study
TC10-1^P_vanA _EV TC10-2^P_vanA_EV TC10-3^P_vanA _EV	transconjugants E. faecium UW261 × 64/3 serially transferred for 400 generations in BHI broth without antibiotics resulting in mixed evolved populations: Van^R, Fus^R, Rif^R	172 kb vanA plasmid	ST21	this study
TC12-1^P_vanA_EV TC12-2^P_vanA_EV TC12-3^P_vanA _EV	transconjugants E. faecium 229710 × 64/3 serially transferred for 400 generations in BHI broth without antibiotics resulting in mixed evolved populations: Van^R, Fus^R, Rif^R	85 kb vanA plasmid	ST21	this study
TC7-1_vanA_EV TC7-2_vanA _EV	fitness-representative strains of the evolved populations TC7^P_EV: Van^R, Fus^R, Rif^R	100 kb vanA plasmid	ST21	this study
TC10-1_vanA _EV TC10-2_vanA _EV TC10-3_vanA _EV	fitness-representative strains of the evolved populations TC10^P_EV: Van^R, Fus^R, Rif^R	172 kb vanA plasmid	ST21	this study
TC12-1_vanA _EV TC12-2_vanA _EV TC12-3_vanA _EV	fitness-representative strains of the evolved populations TC12^P_EV: Van^R, Fus^R, Rif^R	85 kb vanA plasmid	ST21	this study
TC7_vanASS	transconjugant E. faecium (70/90 × 64/3) × 64SS: Van^R, Str^R, Spt^R	100 kb vanA plasmid	ST21	this study
TC10_vanASS	transconjugant E. faecium (UW261 × 64/3) × 64SS: Van^R, Str^R, Spt^R	172 kb vanA plasmid	ST21	this study
TC12_vanASS	transconjugant E. faecium (229710 × 64/3) × 64SS: Van^R, Str^R, Spt^R	85 kb vanA plasmid	ST21	this study

Van, vancomycin; Ery, erythromycin; Str, streptomycin (high level of resistance); Spt, spectinomycin (high level of resistance); Fus, fusidic acid; Rif, rifampicin; Tet, tetracycline; ND, not determined; PSK, post-segregation killing system.

^aPlasmid content present in both donors and transconjugants is described. Several donors contained more than one plasmid; see Werner et al.³⁵