Figure 4.

Reciprocal co-immunoprecipitation of hBest1 and mutant hBest1 in MDCK II cells. MDCK II cells were co-transfected with hBest1-c-myc and an YFP-tagged hBest1 mutant (R218C, W93C and V9M). Co-expressing cells were lysed and hBest1 or mutant hBest1 immunoprecipitated using anti-c-myc or anti-YFP antibodies, respectively, and western blotted using the opposite antibody. Control lanes were loaded with immunoprecipitates prepared from untransfected MDCK II cells. Lysate lanes were loaded using lysates from co-transfected cells.