Fig. 4.

Upregulation of alveolar maintenance programmes by SESN2 inactivation. (A) KGF mRNA expression in WT and Sesn2 KO-MLFs quantified by qRTPCR. (B) Western blot of tenfold concentrated CM (left) and KGF protein concentrations in straight CM measured by ELISA (right) prepared from MLFs before and after PDGF-BB stimulation. (C) A549 cell proliferation in the presence of straight MLF CM ± neutralising anti-KGF antibody estimated by the CyQuant proliferation test. (D) Elastin expression in WT- and KO-MLFs target cells incubated with doubly conditioned A549/WT-MLF and A549/KO-MLF media. Representative western blot (left) and elastin levels in MLFs (right) quantified by densitometry. All results are represented as means ± s.e.m. from n=3 separate experiments. *P<0.05.