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. 2013 Sep 5;6(6):1400–1413. doi: 10.1242/dmm.012229

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Increased Wg levels and spatial distribution at dfmr1 null NMJs. (A) Representative NMJ images co-labeled with neuronal marker (HRP, red) and anti-Wingless (Wg, green) in control (w¹¹¹⁸) and dfmr1 null (dfmr1^50M) wandering third instar muscle 4. Right panels show synaptic boutons at higher magnifications. Scale bars: 15 μm and 3 μm, respectively. Arrows indicate boutons that have been magnified. (B) Quantification of Wg intensity in dfmr1 nulls normalized to w¹¹¹⁸ controls. (C) Analysis of Wg spatial distribution measured as a fraction of the total HRP-labeled NMJ area. (D) Fraction of total HRP-labeled NMJ boutons expressing Wg. Samples sizes are ≥26 animals and ≥52 NMJs for each genotype. Significance is shown as *P<0.05 and ***P<0.001.