Fig. 2.

FREM1 regulates activation of AKT and MAPK upon PDGFC stimulation. (A) Representative western blotting of phosphorylation of AKT and MAPK ERK1/2 in MEFs from WT and bat mouse embryos stimulated with PDGFCC for the indicated time periods. (B) Relative quantification of AKT phosphorylation levels. WT cells 10 minutes after stimulation were assigned a value of 1 and all other samples are standardised against this value. Graph represents average of up to nine WT and 16 bat samples, performed across four independent experiments from at least three different cell lines for each genotype. Black bars: WT; white bars, bat mutant. (C) FREM1 mutation in bat mutants reduces phosphorylation of PDGFRα in response to the addition of exogenous PDGFCC. IP, immunoprecipitation antibody; WB, western blotting antibody. (D) E13.5 WT embryo head skin sections stained for PDGFC (green), PDGFRα (red) and nuclear dye DAPI (blue). Error bars represent standard error of the mean (s.e.m.); *P<0.05, **P<0.01, ***P<0.005.