Skip to main content
. 2013 Nov 6;89(5):899–905. doi: 10.4269/ajtmh.12-0612

Figure 1.

Figure 1.

Polymerase chain reaction amplification of various sources of specimen of patient case 2 (A) were analyzed by electrophoresis on a 1.5% agarose gel and stained with ethidium bromide. Lane BM, bone marrow; lane B, Blood; lane BC, buffy coat; lane S, saliva; lane U, urine; lane T, tissue; lane M,: molecular mass marker (100 basepairs [bp]; lane, P, positive control containing extracted DNA from cultured L. siamensis; lane N1, negative control (no DNA template: double-distilled water); lanes N2–N5: negative control (DNA template from non- infected saliva, urine, blood, and buffy coat, respectively). Comparison of internal transcribed spacer 1 (ITS1) gene sequences amplified from various sources of specimen of the patient 2 (B), Amplified sequences of L. siamensis ITS gene from bone marrow, blood, saliva, urine, and tissue biopsy of patient 2 were assigned GenBank numbers KF227887–KF227892, respectively.