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. 2013 Nov 6;89(5):899–905. doi: 10.4269/ajtmh.12-0612

Figure 2.

Figure 2.

Polymerase chain reaction (PCR) amplification of the internal transcribed spacer 1 (ITS1) gene of Leishmania parasites of the first saliva and urine samples collected from case 4 and her cousin (A) and the second saliva, urine, blood, and buffy coat samples collected from case 4 (B), PCR amplification of case 4 by using primers annealed specifically to small subunit ribosomal RNA of Leishmania parasites (C). Lane B, blood; lane BC, buffy coat; lane S, saliva; lane U, urine; lane M, molecular mass marker (100 basepairs [bp]); lane P, positive control containing DNA from cultured L. siamensis; lane N1, negative control (no DNA template: double-distilled water); lanes N2–N5, negative control (DNA template from non-infected saliva, urine, blood, and buffy coat, respectively).