Table 2.

Primers for real-time quantitative PCR.

Gene	Sequence	Amplicon (bp)	Tm (°C)
COX1	F: 5′-GCCTTTGCTTCAAAACGAGA-3′	105	58
	R: 5′-GGTTGGTTCCTCGAATGTGT-3′
COX2	F: 5′-TCTCCCCTCTCTACGCATTC-3′	161	59
	R: 5′-CAGGTTTTAGGTCGTTTGTTG-3′
COX3	F: 5′-ACTTCACCATCCTCCAAGC-3′	151	59
	R: 5′-TGTCGTAGTAGGCAAACAATAAGG-3′
COX4i1	F: 5′-TCTACTTCGGTGTGCCTTCG-3′	253	59.5
	R: 5′-ACTCATTGGTGCCCTTGTTC-3′
COX5a	F: 5′-GGAGTTGCGTAAAGGGATGA-3′	247	60
	R: 5′-CACTTTGTCAAGGCCCAGTT-3′
COX5b	F: 5′-GGAGGTGGTGTCCCTACTGA-3′	241	59.5
	R: 5′-CAGCCAGAACCAGATGACAG-3′
COX6a1	F: 5′-TCAACGTGTTCCTCAAGTCGC-3′	115	60
	R: 5′-AGGGTATGGTTACCGTCTCCC-3′
COX6b	F: 5′-ATGGCCGAAGACATCAAGAC-3′	250	60
	R: 5′-CAGGAAATGTGCCTTCTGCT-3′
COX6c	F: 5′-AGCGTCTGCGGGTTCATA-3′	154	60
	R: 5′-GCCTGCCTCATCTCTTCAAA-3′
COX7a2	F: 5′-GAGGACCATCAGCACCACTT-3′	234	59.5
	R: 5′-TGGAGACTGGGATGACGAC-3′
COX7b	F: 5′-CGAAGCATTCAGCAAGTGGT-3′	209	59.5
	R: 5′-TGGCATGACTACTGATCTCTCC-3′
COX7c	F: 5′-TCTGCCTTCCGTCTCTGC-3′	145	60
	R: 5′-AGAAAGGAGCAGCAAATCCA-3′
COX8a	F: 5′-TCCTGCTTCGTGTGTTGTCT-3′	70	59
	R: 5′-TCCCGCTTCTTGTAGCTTTC-3′
TFAM	F: 5′-CAGGGCTGCAATTTTCCTAA-3′	141	58
	R: 5′-CCGAAGTGTTTTTCCAGCAT-3′
TFB1M	F: 5′-AAGATGGCCCTTTCGTTTATGG-3′	102	59
	R: 5′-GACTGTGCTGTTTGCTTCCTG-3′
TFB2M	F: 5′-CCAAAACCCATCCCGTCAAAT-3′	135	59
	R: 5′-AAGGGCTCCAAATGTGGAATAAA-3′
SURF1	F: 5′-GGTTCCTGCTTTTAATCCCTGC-3′	119	61
	R: 5′-GATGGGCTCAGCCATGACT-3′
Sp4	F: 5′-TTGCAGCAAGGCCAGCAGACC-3′	130	63
	R: 5′-GCTTCTTCTTTCCTGGTTCACTGCT-3′
NT3	F: 5′-TCACCACGGAGGAAACGCTAT-3′	104	61
	R: 5′-TCAATGGCTGAGGACTTGTCG-3′
Actb	F: 5′-GGCTGTATTCCCTCCATCG-3′	154	59.5
	R: 5′-CCAGTTGGTAACAATGCCATGT-3′