Figure 1. Four families with ASNS mutations.
A. Cosegregation of mutations within each of the four families. Filled symbols represent known or presumed (in the case of aborted fetus) affected individuals. The individuals with a blue star were exome-sequenced.
B. Sanger sequencing confirmation for all three ASNS mutations in the four families. For family D, Sanger sequencing also confirmed inheritance of each mutation from a different parent (compound heterozygote).