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. Author manuscript; available in PMC: 2014 Oct 16.
Published in final edited form as: Neuron. 2013 Oct 16;80(2):10.1016/j.neuron.2013.08.013. doi: 10.1016/j.neuron.2013.08.013

Figure 3. Functional impact of ASNS mutations.

Figure 3

A. RT-PCR to detect ASNS mRNA expression in COS-7 cells transfected with empty, wild-type, or mutant vectors.

B. Western Blots detecting ASNS-FLAG protein abundance, with or without Leupeptin treatment, in the HEK293 transfectants using an anti-FLAG antibody. β-actin was used as a loading control.

See also Figure S2.