Table 2. Analysis of select cytokines secreted by eEC into the apical and basolateral compartments and by eSF.
eECmono | eSFmono | eECco/eSFco | |||
---|---|---|---|---|---|
Cytokine | AS (pg/105 cells) | BS (pg/105 cells) | SS (pg/105 cells) | AS (pg/105 cells) | BS + SS (pg/105 cells) |
CCL2 | 97.42 ± 5.4 | 72.76 ± 1.4a | 492.9 ± 17.9b | 70.4 ± 2.3c | 1,695.1 ± 31.8d |
CCL3 | 5.76 ± 0.3 | ND | ND | 16.23 ± 0.7c | ND |
CCL4 | 1.0 ± 0.2 | ND | ND | 2.32 ± 0.1c | ND |
CCL5 | ND | ND | ND | ND | ND |
CCL7 | ND | ND | 13.8 ± 1.0 | ND | 20.11 ± 0.14 |
CSF2 | 13.7 ± 5.1 | 13.7 ± 1.1 | 1.0 ± 0.6b | 23.2 ± 12.0 | 5.3 ± 1.7d |
CX3CL1 | 56.27 ± 5.3 | ND | 46.3 ± 7.0 | 43.18 ± 1.7 | 17.7 ± 1.76d |
IFNG | ND | ND | ND | ND | ND |
IL1A | 47.4 ±11.1 | ND | ND | 42.3 ± 35.1 | ND |
IL1B | ND | ND | ND | ND | ND |
IL2 | ND | ND | ND | ND | ND |
IL4 | 12.4 ± 2.3 | ND | ND | 11.6 ± 2.4 | ND |
IL5 | ND | ND | ND | ND | ND |
IL6 | 113.5 ± 38.5 | 15.6 ± 5.4a | 22.6 ± 18.4b | 57.9 ± 16.1 | 43.5 ± 19.0 |
IL8 | 807.0 ± 140.1 | 573.75 ± 213.2 | 128.3 ± 24.88b | 627.38 ± 70.3 | 1,085.7 ± 501 |
IL10 | ND | ND | ND | ND | ND |
TNFA | 23.55 ± 4.22 | 9.55 ± 4.92a | ND | 17.75 ± 7.1 | ND |
Note: AS = apical; BS = basolateral; eFS = SS; ND =nondetectable.
P< .05 between eECmono AS and eECmono BS.
P< .05 between eECmono AS + BS and eSFmono SS.
P< .05 between eECmono AS and eECco/eSFco AS.
P< .05 between eECco/eSFco SS + BS and eECmono BSmono + eSFmono.