Table 1. Primer sequences used to amplify USH2A exons 20 and 70 from gDNA samples.
Primer Name | Sequence (5′-3′) | Tm (°C) | Direction | Amplicon Length (bp) |
---|---|---|---|---|
USH2A Ex20-F |
TCCTAATGGTGGTTGGCAAT |
60 |
Sense |
382 |
USH2A Ex20-R |
AGTGAGGGAGGAGAAGACAAA |
58 |
Antisense |
382 |
USH2A Ex70-F |
CTACAGCGAGCTGTGGTTCA |
60 |
Sense |
362 |
USH2A Ex70-R | GCAGCCAAAGTTGAGAAAGC | 60 | Antisense | 362 |