Table 2. Post-translational modifications identified for proteins in SOIcs and SOIar for 12-week-old OXYS lens.
| Spot Nº (Crystallin) | Sequence coverage, % |
Abundance, % |
Modification |
|||||
|---|---|---|---|---|---|---|---|---|
| Wistar | OXYS | Oxidation (M) | Acetylation (N-term) | Deamidation (N, Q) | Phosphory-lation (S, T) | |||
|
Cataract-specific spots of interest (SOIcs) | ||||||||
| 2 (αA) |
59 |
ND |
0.3±0.3 |
M1 |
M1 |
T4 |
||
| 3 (αA) |
87 |
0.17±0.09 |
0.83±0.16 |
M1, M138 |
M1 |
T4 |
||
| 4 (αA) |
89 |
0.13±0.14 |
0.61±0.24 |
M1, M138 |
M1 |
T4 |
||
| 5 (αA) |
89 |
1.26±0.37 |
3.06±1.28 |
M1 |
M1 |
N123 |
T4 |
|
| 9 (αA) |
82 |
0.46±0.35 |
1.32±0.54 |
M1, M138 |
M1 |
N123, Q126 |
T4 |
|
| 10 (αA) |
71 |
1.29±0.37 |
3.58±0.86 |
M1, M138 |
M1 |
T4 |
||
| 11 (αA) |
78 |
0.34±0.17 |
0.78±0.39 |
M1 |
M1 |
N123, Q126 |
T4 |
|
| 13 (αA) |
48 |
ND |
0.30±0.07 |
M1 |
M1 |
T4 |
||
| 21 (αA) |
87 |
0.84±0.25 |
1.59±0.57 |
M1 |
M1 |
T4 |
||
| 24 (αB) |
65 |
ND |
0.30±0.14 |
M1 |
M1 |
S19 |
||
| 36 (αB) |
73 |
0.49±0.27 |
0.85±0.13 |
M1, M68 |
M1 |
N146 |
||
| 46 (αB) |
65 |
ND |
0.19±0.05 |
M1 |
M1 |
N146 |
S19 |
|
| 56 (αB) |
70 |
ND |
0.52±0.13 |
M1 |
M1 |
N146 |
||
| 8 (βA4) |
98 |
ND |
0.23±0.05 |
Q112, N114, Q187, Q189 |
||||
| 22 (βA4) |
96 |
ND |
0.66±0.37 |
Q112, N114 |
||||
| 18 (βB1) |
72 |
ND |
0.13±0.07 |
M135, M162, M224 |
||||
| 32 (βB1) |
67 |
ND |
0.91±0.30 |
M135, M162 |
||||
| 61 (βB2) |
93 |
0.86±0.33 |
1.71±0.71 |
N174, Q185 |
||||
|
Age-related spots of interest (SOIar) | ||||||||
| 20 (βA4) |
91 |
4.51±1.80 |
2.90±0.86 |
M132 |
Q63, Q65, Q66, Q112, N114, Q187, Q189 |
|||
| 80 (γA) |
63 |
4.61±2.51 |
2.17±1.39 |
M136, M160 |
||||
| 86 (γA) |
66 |
11.5±2.61 |
5.17±1.11 |
M136, M160, M124 |
||||
| 84 (γC) |
58 |
4.32±1.57 |
1.93±0.96 |
M102 |
||||
| 73 (γE) | 58 | 6.89±1.67 | 3.65±1.98 | M44, M102, M160 | N50, N161 | |||
ND (not detected) – protein spots are not found in the 12-week-old Wistar 2-DE maps.