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. 2012 Dec 31;2012:126170. doi: 10.6064/2012/126170

Table 2.

Amount of HRP attached protein (see (5)) on untreated and PIII-treated UHMWPE and on copolymer of polyethylene and methancrylic acid (co-PE-MMA) before and after washing in detergents. The HRP protein attachment was done on PIII-treated sample in air, in nitrogen at low pressure (30 Torr), and high pressure (760 Torr). Carbonyl group relative concentration is measured by intensity ratio of 1691 cm−1 line and 1462 cm−1 line in FTIR ATR spectra. The average error bar for the protein amount is 0.0010.

Sample description Amount of attached after incubation Amount after SDS Amount after Triton Amount after Tween 20 C=O concentration before attachment
Untreated 0.0087 0.0041 0.0018 0.0023 ~0
PIII, in air 0.0198 0.0143 0.0142 0.0167 0.14
PIII, in N2, 30 Torr 0.0182 0.0161 0.0106 0.0100 ~0
PIII, in N2, 760 Torr 0.0255 0.0147 0.0186 0.0180 ~0
co-PE-MMA, pressed 0.0086 0 0.0033 0.0024 0.46
co-PE-MMA, pressed and ethanol washed 0.0057 0 0.0014 0.0029 0.53