Table 2.
Amount of HRP attached protein (see (5)) on untreated and PIII-treated UHMWPE and on copolymer of polyethylene and methancrylic acid (co-PE-MMA) before and after washing in detergents. The HRP protein attachment was done on PIII-treated sample in air, in nitrogen at low pressure (30 Torr), and high pressure (760 Torr). Carbonyl group relative concentration is measured by intensity ratio of 1691 cm−1 line and 1462 cm−1 line in FTIR ATR spectra. The average error bar for the protein amount is 0.0010.
| Sample description | Amount of attached after incubation | Amount after SDS | Amount after Triton | Amount after Tween 20 | C=O concentration before attachment |
|---|---|---|---|---|---|
| Untreated | 0.0087 | 0.0041 | 0.0018 | 0.0023 | ~0 |
| PIII, in air | 0.0198 | 0.0143 | 0.0142 | 0.0167 | 0.14 |
| PIII, in N2, 30 Torr | 0.0182 | 0.0161 | 0.0106 | 0.0100 | ~0 |
| PIII, in N2, 760 Torr | 0.0255 | 0.0147 | 0.0186 | 0.0180 | ~0 |
| co-PE-MMA, pressed | 0.0086 | 0 | 0.0033 | 0.0024 | 0.46 |
| co-PE-MMA, pressed and ethanol washed | 0.0057 | 0 | 0.0014 | 0.0029 | 0.53 |