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. 2013 Nov 7;8(11):e78478. doi: 10.1371/journal.pone.0078478

Figure 6. GDF11 does not induce neuronal nor astrocytic differentiation.

Figure 6

(A) Cor-1 cells incubated with 50 ng/ml of GDF11 for 48 hr are negative for either the early neuronal marker Tuj1 (left) or the glial marker GFAP (centre). Incubation with 10 ng/ml of BMP4 for 48 hr induces astrocytic differentiation revealed by positive GFAP immunoreactivity. Hoechst dye (blue) was used to stain cell nuclei. Scale bars are 40 µm. Consistent with the immunocytochemistry results, Tuj1 (B) and GFAP (C) cannot be detected by Western blot in lysates of Cor-1 cells exposed to either 25 or 50 ng/ml GDF11 for 24 or 48 hr. As positive controls, Tuj1 is detected in Cor-1 cells plated on laminin and maintained for 48 hr in medium lacking EGF (B, first lane on the left) while GFAP is detected in Cor-1 cells undergoing astrocytic differentiation after incubation with BMP4 for 48 hr (C, first lane on the left).