Figure 2. RPA, bound to ssDNA, interacts with DNA polymerase D and DNA primase.

The catalytic subunit of the DNA primase (A), PolD (B) and PolB (C) were injected at different concentrations (16.67 nM, 50 nM, 150 nM and 450 nM) on a chip coated with the assembly of a 32mer ssDNA bound by RPA. Signals reported (black curves) are already subtracted from a RPA dissociation curve and data were fitted using a 1:1 binding reaction model (red curves).