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. 2013 Nov 7;8(11):e79707. doi: 10.1371/journal.pone.0079707

Figure 3. PCNA interacts with Mre11/Rad50 complex.

Figure 3

10 µg of HIS fusion Mre11/Rad50 immobilized on Co2+ magnetic beads were incubated with 0.5 µg of PCNA in absence or presence of DNase I (Pulldown Nuc- or Pulldown Nuc+). Control lanes consisted in the Co2+ beads only (1) or incubated with either the complex Mre11/Rad50 (2) or the PCNA (3). Bait and prey proteins were analysed by Western blot, and probed with PCNA or HIS antibodies.