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. 2013 Nov 7;8(11):e79707. doi: 10.1371/journal.pone.0079707

Figure 4. Pab0431 interacts with PCNA and displays a putative C-terminal PIP motif.

Figure 4

A, 5 μg of histidine-tagged Pab0431 bound to magnetic beads (lane pull-down), and a negative control that consists of the beads only, were incubated with 16 mg of P. abyssi cellular extract. Control lanes contained 0.33 μg of cell free extract and 8.75 ng of PCNA. The presence of PCNA on the blot was revealed using anti-PCNA antibody. B, Pab0431 has a canonical C-terminal PIP box motif when compared to the consensus sequence (x and h respectively refer to any amino acids and hydrophobic residues) and various sequences of proteins from P. abyssi known to interact with the PCNA through the PIP motif. The position of the PIP box within the sequences is indicated.