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. Author manuscript; available in PMC: 2013 Nov 7.
Published in final edited form as: Dev Biol. 2011 Jan 14;351(2):277–286. doi: 10.1016/j.ydbio.2011.01.008

Fig. 3.

Fig. 3

Abnormal expression of the maternally expressed genes Ascl2 and Phlda2 in Del7AI/+ placentae. The expression patterns of Ascl2 (A), Tpbpa (B) and Cdkn1c (C) were analyzed by ISH on frozen sections of wild type (+/+) and maternal deletion (Del7AI/+) placentae collected at E9.5. Multiple sections from two placentae of each genotype were assessed and representative sections are shown. Sense probes are not shown. The blue stain shows gene expression. Nuclear fast red was used as the counterstain. (D) PHLDA2 IHC at E13.5. The brown stain shows protein expression in the labyrinth. Scale bar: 0.5 mm. sp: spongiotrophoblast layer, lab: labyrinthine layer, d: decidua.