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. 2013 Aug 29;30(5):2042–2048. doi: 10.3892/or.2013.2708

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Copyright © 2013, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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mRNA expression of ODC, tumstatin, endostatin and VEGF in HEK293 cells after treatment. (A) A 1,286 bp band representing the ODC gene, (B) a 750 bp fragment representing the tumstatin gene, (C) a 261 bp fragment representing endostatin and (D) 156 bp fragment representing VEGF were amplified by RT-PCR and visualized. β-actin was also amplified as an internal control. Lane M, DL-2000 marker; lane 1, PBS-treated group; lane 2, mock transfectants; lane 3, ODC transfectants; lane 4, ODC transfectants transfected with pcDNA-ODCr; lane 5, putrescine-treated group.