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Western blot analysis was performed to detect the expression of ODC and tumstatin gene in cells after treatment. Lane 1, PBS-treated group; lane 2, mock transfectants; lane 3, ODC transfectants; lane 4, ODC transfectants transfected with pcDNA-ODCr; lane 5, putrescine-treated group. β-actin was detected with an anti-β-actin antibody as an internal control.
