Figure 5.

Analysis of the tumstatin promoter activity. (A) Tumstatin gene promoter activity in HEK293 cells. pGL-tumstatin (2.2 kb) and pRL-TK were co-transfected into HEK293 cells to detect whether the amplified fragment possesses promoter activity. pGL3-basic and pGL3-control were used as negative control and positive control, respectively. (B) Dual-luciferase reporter assay was used to detect the effects of the treatment on promoter activity of tumstatin gene. Each bar represents the mean ± SD of three experiments.