Table 1. Primers used for amplifying the cDNA and genomic DNA of c-type and g-type lysozymes and expression analysis in Asian seabass.

Primer name	Primer sequence (5′-3′)	Annealing temperature (°C)	Application
(c-type)-A	GCAGCGAGCTTCTGACTGATGAT	60	cDNA 3′ RACE
(c-type)-B	TTACACCCCACAACCTGACACATA	60	cDNA 5′ RACE
g(c-type)-F	ACATTTGATCCAGCAGAGAATACA	57	Amplified genomic DNA
g(c-type)-R	CACCCCACAACCTGACACA
r(c-type)-R	GCAGCGAGCTTCTGACTGATGAT	55	Real-time PCR
r(c-type)-F	ATTACACCCCACAACCTGACACATAG
e(c-type)-F	CCGAATTCCATGAGGGGTCTGCT	57	Expression in E. coil
e(c-type)-R	CGAGCTCAGTTACACCCCACAACC
snp(c-type)-F	CATACTTATTAGCGTCCTTGC	55	SNP PCR
snp(c-type)-R	CTATCTACAGCCACATCCACC
(g-type)-A	AGAGTCCAGGGCTGGAAAT	60	cDNA 3′ RACE
(g-type)-B	TGTACCACTGAGCTCTGGC	60	cDNA 5′ RACE
g(g-type)-F	ATGGGTGGGTGCTTTTACCTTCTT	57	Amplified genomic DNA
g(g-type)-R	AGCCGTTGCTTTTGTACCACTGAG
r(g-type)-F	AGAGTCCAGGGCTGGAAAT	55	Real-time PCR
r(g-type)-R	TGTACCACTGAGCTCTGGC
e(g-type)-F	CGAATTCCATGGGTTATGGAGACATCA	58	Expression in E. coil
e(g-type)-R	TCTCGAGCTAAAAGCCGTTGCTTTTG
snp(g-type)-F	TTGCGATAATGACTGACTAATG	55	SNP PCR
snp(g-type)-R	TCAACCTACAGAAATGGGAAT

Note: Restriction site is underline