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. 2013 Nov 7;8(11):e79359. doi: 10.1371/journal.pone.0079359

Figure 2. Expression of ICK/MAK/MOK proteins in young adult and embryonic mouse tissues.

Figure 2

(A) Equal amount of total proteins (50 µg) extracted from various frozen tissues of young adult C57BL/6J mice (8 week-old) were Western blotted against antibodies recognizing ICK, MAK, and MOK respectively. Western blot signal of ERK2, closely related to ICK/MAK/MOK, was also shown as a control to indicate a universal expression pattern in various young adult mouse tissues. (B) Equal amount of total proteins (50 µg) extracted from frozen tissues (lung, liver, kidney and intestine) of mouse embryos of E13.5 and E16.5 were Western blotted against antibodies recognizing ICK, MAK, and MOK respectively. Western blot signals of ERK1/2 were shown as controls to indicate a universal expression pattern in various mouse embryonic tissues.