Figure 3. AcSDKP restored the proliferation of U87-MG cells through Akt activation.

(A) Effect of S17092 on the phosphorylation of Akt (Ser⁴⁷³) and ERK1/2 in U87-MG cells. Cells were incubated with different concentrations of S17092 for 2 h and analyzed by Western blot with antibodies against p-Akt and p-ERK1/2 proteins. (B) Effect of AcSDKP on the phosphorylation of Akt and ERK1/2 in S17092-treated U87-MG cells. Cells were pretreated with 100 μg/ml of S17092 for 2 h and then combined with AcSDKP at the indicated concentrations for 2 h. The graph represents the relative level of Akt and ERK1/2 phosphorylation compared with the control group. Similar results were obtained from three independent experiments. The ratio of band intensities was calculated after normalizing the phospho-protein signals with the total protein signals. The calculations were done using NIH image software.