Figure 4. Effect of wortmannin, a PI3K inhibitor, on AcSDKP-induced Akt phosphorylation and U87-MG proliferation.

Cells were pretreated with 100 μg/ml S17092 for 90 min and then with 10 μM wortmannin for additional 30 min before addition of different concentrations of AcSDKP in the presence of 100 μg/ml S17092. (A) Effect of wortmannin after co-treatment for 2 h with POPi and AcSDKP was analyzed by Western blot for p-Akt and Akt proteins. (B) Cell proliferation was determined after co-treatment for 24 h with POPi and AcSDKP by [³H]-thymidine incorporation assays performed in triplicate. Representative results are shown from three independent experiments.