Table II.

Toll-like-receptor-4 (TLR4) competing assay.

Treatment	Group
	N	D	XP	L100	L200	T1	T2
Der p 2a	-	+	+	+	+	+	+
XQLTb	-	-	+	+	+	+	+
Treatment combined with XQLT	-	-	-	LPS 100 ng/ml	LPS 200 ng/ml	+sTLR4 1 μg/ml	+sTLR4 2 μg/ml

^aDer p 2 (75 μg/ml) for 24 h;

^bXiao-Qing-Long-Tang (XQLT; 1 mg/ml) pulse 1 h. Briefly, lipopolysaccharide (LPS) or exogenous recombinant soluble TLR4 (sTLR4) was used to pretreat XQLT at room temperature. The mixture of XQLT and sTLR4 or LPS was then added to the cell culture. One hour subsequently, the previously mentioned XQLT mixture and medium were washed-out. Cells were then stimulated with Der p 2 for the next 24 h. The supernatants and total cell protein were collected 24 h later. N, negative control; D, positive control; XP, XQLT treatment.