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. 2013 Sep 2;6(5):1189–1193. doi: 10.3892/etm_2013.1281

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Copyright © 2013, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Figure 1. — Observation of HPMCs. Observation of HPMCs under a microscope at (A) day 2 after primary culture (magnification, ×200; observed under light microscope, no staining) and (B) day 14 after primary culture (magnification, ×200; observed under light microscope, no staining). Immunofluorescence showed (C) vimentin-positive HPMCs (magnification, ×400; immunofluorescence staining) and (D) cytokeratin-positive HPMCs (magnification, ×400; immunofluorescence staining). (E) Electron microscopy showed an HPMC in PD effluent culture (magnification, ×10,000; gilded with ion sputter). (F) Transmission electron microscopy of an HPMC in PD effluent culture (magnification, ×4,000; gilded with ion sputter). (G) Mixed mesothelium (magnification, ×200; observed under light microscope, no staining). (H) Fibroblast-like mesothelium (magnification, ×200; observed under light microscope, no staining). (A–G) HPMCs were from the PD start group; (H) HPMCs were from the PD >6 months group. HPMCs, human peritoneal mesothelial cells; PD, peritoneal dialysis.