Figure 3. sry-α and spt induce the same loss of function phenotype.

(A) Surface views of furrows and nuclei, detected in living embryos (membrane Gap43-mCherry, green; nuclear Histone-GFP, purple), following the indicated sry-α^RNAi or spt^RNAi treatment. Multinucleate cells highlighted (orange nuclei) in corresponding segmented images. (B) Quantification of multinucleation phenotypes. Each point represents one embryo with ≥150 nuclei analyzed (n = 12 embryos per condition). (C) Frequency of multinucleation for embryos following the indicated RNAi treatment (n≥6,000 nuclei from 30 embryos per treatment; mean ± s.e.m.). Student's t-test was performed to calculate P values as shown in (B, C).